Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability
نویسندگان
چکیده
منابع مشابه
Ofatumumab Monoclonal Antibody Affinity Maturation Through in silico Modeling
Background: Ofatumumab, an anti-CD20 mAb, was approved in 2009 for the treatment of chronic lymphocytic leukemia. This mAb acts through immune-mediated mechanisms, in particular complement-dependent cytotoxicity and antibody-dependent cellular cytotoxicity by natural killer cells as well as antibody-dependent phagocytosis by macrophages. Apoptosis induction is another mechanism of this antibody...
متن کاملSomatic hypermutation maintains antibody thermodynamic stability during affinity maturation.
Somatic hypermutation and clonal selection lead to B cells expressing high-affinity antibodies. Here we show that somatic mutations not only play a critical role in antigen binding, they also affect the thermodynamic stability of the antibody molecule. Somatic mutations directly involved in antigen recognition by antibody 93F3, which binds a relatively small hapten, reduce the melting temperatu...
متن کاملAntibody affinity maturation using bacterial surface display.
A quantitative system for screening combinatorial single-chain Fv (scFv) antibody libraries was developed utilizing surface display on Escherichia coli and fluorescence-activated cell sorting (FACS). This system was employed to isolate clones with high-affinity to a fluorescently-labeled hapten from libraries constructed by randomizing heavy and light-chain residues in the anti-digoxin 26-10 de...
متن کاملEffects of age on antibody affinity maturation.
The elderly are more susceptible to infectious diseases. Mortality and morbidity from infections increase sharply over the age of 65 years. At the same time, the efficacy of vaccinations in the elderly is decreased. The elderly also have an increased incidence of cancer and inflammatory diseases. All the above indicate an age-related dysregulation of the immune system. Evidence suggests that th...
متن کاملRetraction for Wang et al., Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation.
BIOCHEMISTRY Retraction for “Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation,” by Feng Wang, Shiladitya Sen, Yong Zhang, Insha Ahmad, Xueyong Zhu, Ian A. Wilson, Vaughn V. Smider, Thomas J. Magliery, and Peter G. Schultz, which was first published February 25, 2013; 10.1073/ pnas.1301810110 (Proc Natl Acad Sci USA 110:4261–4266). The undersigned autho...
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ژورنال
عنوان ژورنال: Cell Reports
سال: 2019
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2019.08.056